Organisers

Johan den Dunnen: Leiden University Medical Center, Netherlands
Judith Boer: Leiden University Medical Center, Netherlands
Edwin Cuppen: Hubrecht Institute and Utrecht Universitair Medisch Centrum, Netherlands
Ivo Gut: Centre National de Génotypage, Paris, France
Paul Flicek: European Bioinformatics Institute, United Kingdom
Joris Veltman: Radboud University Nijmegen Medical Centre, Netherlands
Xavier Estivill: Pompeu Fabra University, Barcelona, Spain
Matthew Hestand: Leiden University Medical Center, Netherlands
Terry Vrijenhoek: Radboud University Nijmegen Medical Centre, Netherlands

Introduction

Next generation sequence (NGS) technologies enable high-throughput sequencing for applications including de novo sequencing and re-sequencing of (subsets of) genomic DNA, and whole transcriptome and transcript-tag sequencing. From the introduction of these new technologies, the developments have been very fast. While the technologies are being improved further, and even next-next generation sequencing will become available soon, pioneer researchers have started to successfully apply NGS to their research questions. However, because of the rapid developments, many scientists who are thinking about using NGS for their research have a need for information and sharing of (first) experiences. Furthermore, recent results show applications emerging in fields that are not immediately obvious, including evolution, environment, forensics and archaeology.

We aim at bringing together the experts in the field to present their work and share their experiences with European researchers. This will accelerate the diffusion and exchange of information among European researchers, including those from regions where NGS is not yet being used.

Draft Programme

•  Re-sequencing

Application of 'next-generation' sequencing technologies to microbial genetics: David Studholme, The Sainsbury Laboratory, Norwich, UK
1000 Genomes: Paul Flicek, Wellcome Trust Sanger Institute, Hinxton , UK
Whole exome: Jay Shendure, Dept. of Genome Sciences, Univ of Washington, Seattle
In vitro mutagenesis: Edwin Cuppen, Hubrecht Institute, Utrecht, NL

•  De novo sequencing and assembly

Large-scale sequence analyses of Atlantic cod: Steinar Johansen, Dept. of Molecular Biotechnology, Institute of Medical Biology, University of Tromsø, Norway
Bacterial metagenomics: Sacha van Hijum, NIZO Food Research, Wageningen, NL
Next-generation sequencing and metagenomic analysis: a universal diagnostic tool in plant virology: IP Adams, Central Science Laboratory, Sand Hutton, York, UK
Evolution: Svante Pääbo, Dept. of Evolutionary Genetics, Max Planck Institute for Evolutionary Anthropology, Leipzig , Germany or: 0Eske Willerslev, Ancient DNA and Evolution Group, Department of Biology, Univ of Copenhagen , Denmark

•  Gene expression and structure

Tag-based (SAGE & CAGE): Matthias Harbers, RIKEN, Japan
Gene expression profiling of the host response to Mycobacterium bovis infection in cattle: David Machugh, Animal Genomics Laboratory, Univ College Dublin, Ireland and/or:
mRNA-Seq whole-transcriptome analysis of a single cell: MA Surani, Wellcome Trust-Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, Univ of Cambridge, UK
miRNA: Edwin Cuppen, Edwin Cuppen, Hubrecht Institute, Utrecht, NL

•  Personalised genomes

Variation – 1000 Genomes: Richard Durbin, Wellcome Trust Sanger Institute, Hinxton , UK
Structural variants: Matthew Hurles, Wellcome Trust Sanger Institute, Hinxton , UK
The diploid genome sequence of an Asian individual: Ruiqiang Li, Beijing Genomics Institute, Shenzhen , China

•  Future developments

General: Ivo Gut, Centre National de Génotypage, Paris, France
Single Molecule Real Time DNA sequencing: Pacific Biosciences, Menlo Park, California,USA
Direct RNA sequencing: P Milos, Helicos BioSciences Corporation, Cambridge, Massachusetts, USA
Single-molecule nanopore DNA sequencing: Hagan Bayley, Dept. of Chemistry, Univ of Oxford, UK

•  Demonstrations

Use of 1000 genome sequences
Visualisation including Genome Browsers
Public data repositories: GEO, ArrayExpress, SRA, EGA
Analysis software practicals/demonstrations

Confirmed List of Speakers

• Sacha van Hijum, Radboud University Medical Centre, Nijmegen, Netherlands
• Eske Willerslev, Ancient DNA and Evolution Group, Univ of Copenhagen, Denmark
• Ivo Gut, Centre National de Génotypage, Paris, France
• Chris Tyler-Smith, The Wellcome Trust Sanger Institute, UK
• Mike Wilson, Cancer Research UK, University of Cambridge, UK
• Matthias Harbers, DNAFORM, Japan
• Edwin Cuppen, Hubrecht Institute, Utrecht, Netherlands
• Barbara Wold, California Institue of Technology, USA
• Joris Veltman, Radboud University Medical Centre, Nijmegen, Netherlands
• Debbie Nickerson, University of Washington, Seattle, USA
• Ewan Birney, EBI, Hinxton, UK
• Guy Cochrane, EBI, Hinxton, UK
• Ruiqiang Lui, Beijing Genomics Insitute, China
• Evan Eichler, University of Washington, Seattle, USA

Venue

The conference will take place at the Leiden University Medical Center (LUMC) which is located at walking distance of Leiden Central Railway Station, which is 20 minutes from Amsterdam Airport (Schiphol) by direct train.

Registration

Registration is now closed.